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clear plastic cones
Plants
Nine to ten day old plants of TAM 107 wheat were used for these
experiments. Plastic cones (2.5 cm diameter × 16 cm deep)
were filled with soil and planted with three wheat seeds in each.
After planting, cones were placed in plastic racks set in plastic
trays filled with water for 48 h in the greenhouse. During this
period, the cones drew up enough water from the tray to support
plant growth throughout the experiment. Three to 4 days after
germination, plants were thinned to leave a single seedling in
each cone. Plants were grown for 9-10 days until they reached
the 2-3 leaf stage, then cut to a height of 10 cm in order to
facilitate repeated observations of aphids that are easily dislodged
from tall plants.
Experiments
Adult apterae of all three species were collected from their respective
stock colonies and placed on wheat seedlings (2-3 per plant) to
reproduce for a period of 24 h, yielding a synchronous cohort
of 12 ± 12 h old first instar nymphs. Seedlings in individual
cones (replicates) were each infested with two first instar nymphs
on the stem and then covered with a ventilated clear plastic cylinder
(2.3 cm diameter × 31 cm tall) and placed back in the rack.
The six experimental treatments consisted of three heterospecific
pair wise combinations of nymphs with three conspecific pair combinations
serving as controls. There were twenty replications of each treatment.
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plastic cones
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