By Application:    Architectural Laboratory Water Treatment Marine Air Handling

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The reactor was operated at 132 rpm (a surface velocity of 0.44 m s?1). The reactor volume was 500 ml, and flow through the reactors was 1 liter day?1. The water in the reservoir was replaced with fresh river water throughout the experimental period on an 8-day schedule (days 1, 3, 5, 8, etc.). Biofilms were grown on polycarbonate strips in rotating annular bioreactors, as described previously. In brief, the reactors were constructed using a rotating inner solid polycarbonate cylinder machined to allow insertion of 12 removable polycarbonate slides; the outer cylinder was a commercially available 1-liter glass jar with a threaded mouth (38). Mixing in the reactor was via the rotation of the inner cylinder and the circulation of water through the reactor from top to bottom. The experimental setup included a temperature-controlled (17 ± 2°C) 10-liter reservoir for the raw water, a peristaltic pump for circulating the water, and the rotating annular reactor with motor and control for adjusting the speed of the rotating inner cylinder. All reactor components were surface disinfected with 1.2% sodium hypochlorite for 1 h prior to use.
The biofilms were grown for 8 weeks at the dates described in Table 1. Each fall, two replicate bioreactors were operated for each of the following conditions, in the absence and in the presence of hexadecane (1 ppb): river water alone (no nutrient addition, control); river water amended with glucose at 67 ?mol/liter of carbon; river water amended with NH4Cl at 80 ?mol/liter of nitrogen; river water amended with KH2PO4 at 5 ?mol/liter of phosphorus; and river water amended with a combination of all three nutrients at the concentrations described above (44).

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